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Hermes‐mediated germ‐line transformation of the Mediterranean fruit fly Ceratitis capitata
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Personen und Körperschaften: | , , , , , , , , |
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Titel: |
Hermes‐mediated germ‐line transformation of the Mediterranean fruit fly Ceratitis capitata |
In: | Insect Molecular Biology, 10, 2001, 2, S. 155-162 |
veröffentlicht: |
Wiley
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Umfang: | 155-162 |
ISSN: |
0962-1075 1365-2583 |
DOI: | 10.1046/j.1365-2583.2001.00250.x |
Zusammenfassung: | <jats:title>Abstract</jats:title><jats:p>We report the use of the <jats:italic>Hermes</jats:italic> transposable element for germ‐line transformation of the Mediterranean fruit fly, <jats:italic>Ceratitis capitata</jats:italic>. <jats:italic>Hermes</jats:italic> was able to genetically transform this insect at an estimated frequency between 0.6 and 1.1%, which is comparable to the transformation frequencies obtained for this species when using other transposable elements. <jats:italic>Hermes</jats:italic> integrates into the medfly genome by a cut‐and‐paste mechanism and the sequences integrated into the genome are delimited by the terminal nucleotides of the <jats:italic>Hermes</jats:italic> inverted terminal repeats. Integration resulted in the generation of 8 bp target site duplications, the sequences of which conformed to the target site duplications generated by <jats:italic>hAT</jats:italic> element transposition in insects. The <jats:italic>Hermes</jats:italic> element is one additional genetic tool that can be deployed in manipulating and characterizing the medfly genome.</jats:p> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |