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Arteriolization of Capillaries and FGF‐2 Upregulation in Skeletal Muscles of Patients with Chronic Peripheral Arterial Disease
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Titel: |
Arteriolization of Capillaries and FGF‐2 Upregulation in Skeletal Muscles of Patients with Chronic Peripheral Arterial Disease |
In: | Microcirculation, 12, 2005, 6, S. 527-537 |
veröffentlicht: |
Wiley
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Umfang: | 527-537 |
ISSN: |
1073-9688 1549-8719 |
DOI: | 10.1080/10739680591003413 |
Zusammenfassung: | <jats:title>ABSTRACT</jats:title><jats:p><jats:bold>Objective:</jats:bold> Microvascular changes in ischemic skeletal muscle are described derived from patients with long‐lasting peripheral arterial disease (PAD).</jats:p><jats:p><jats:bold>Methods:</jats:bold> Skeletal muscles from the lower limb of 17 patients (obtained after amputation) with chronic PAD and 4 asymptomatic controls (obtained from biopsies after bypass surgery) were evaluated by electron microscopy and immunohistochemistry.</jats:p><jats:p><jats:bold>Results:</jats:bold> The capillaries in skeletal muscles of PAD patients were surrounded by a more than 1μ m‐thick coat, which was positively stained for basement membrane pericapillary coat collagen type IV. Thickness of the coat correlated with presence of PAD (<jats:italic>p</jats:italic> < .0001), and less strongly with diabetes mellitus (<jats:italic>p</jats:italic> = .023) and age of patients (<jats:italic>p</jats:italic> = .019). The majority of the capillaries in skeletal muscles of PAD patients (71.1 ± 15.3%) were covered with cells positive for smooth muscle cell actin (sma) as compared to samples from asymptomatic controls (22.8% ± 9.6%; <jats:italic>p</jats:italic> < .0001) suggesting advanced arteriolization. Semiquantitative analysis revealed that patients with PAD demonstrate a higher expression of FGF‐2 in capillary endothelial cells (67.8 ± 17.5%) as compared to controls (10.2 ± 8.4%; <jats:italic>p</jats:italic> < .0001), whereas VEGF immunoreactivity was only occasionally present in extravascular cells.</jats:p><jats:p><jats:bold>Conclusion:</jats:bold> Thickened collagen type IV‐positive basement membranes in combination with a significant increase in sma‐coverage indicate arteriolization of capillaries characteristic for chronic PAD, what may be related to high FGF‐2 expression in capillary endothelial cells.</jats:p> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |