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Modulation of 2′‐5′oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium
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Titel: |
Modulation of 2′‐5′oligoadenylate synthetase by environmental stress in the marine sponge Geodia cydonium |
In: | Environmental Toxicology and Chemistry, 16, 1997, 7, S. 1403-1409 |
veröffentlicht: |
Wiley
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Umfang: | 1403-1409 |
ISSN: |
0730-7268 1552-8618 |
DOI: | 10.1002/etc.5620160712 |
Zusammenfassung: | <jats:title>Abstract</jats:title><jats:p>Recently we established the presence of relatively high amounts of 2′‐5′oligoadenylates (2′‐5′A) and 2′‐5′oligoadenylate synthetase (2′‐5′A synthetase) in the marine sponge <jats:italic>Geodia cydonium.</jats:italic> Here we determined by applying radioimmunoassay and high‐performance liquid chromatographical methods that the concentration of 2′‐5′A and the activity of 2′‐5′A synthetase change following exposure of <jats:italic>G. cydonium</jats:italic> tissue to environmental stress. The 2′‐5′A content and the activity of 2′‐5′A synthetase, present in crude sponge extract, increase by up to three‐fold after treating sponge cubes for 2 h with natural stressors including heat shock (26°C), cold shock (6°C), pH shock (pH 6), and hypertonic shock and subsequent incubation for 18 h under ambient conditions (16°C). No response was observed after exposure of sponges to an alkaline (pH 10) or hypotonic environment. Similar changes have been found for the expression of heat shock protein HSP70 in <jats:italic>G. cydonium.</jats:italic> These results show that 2′‐5′A in sponges may be useful as a novel biomarker for environmental monitoring.</jats:p> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |