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Cucurbitacin B acts a potential insect growth regulator by antagonizing 20‐hydroxyecdysone activity
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Titel: |
Cucurbitacin B acts a potential insect growth regulator by antagonizing 20‐hydroxyecdysone activity |
In: | Pest Management Science, 74, 2018, 6, S. 1394-1403 |
veröffentlicht: |
Wiley
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Umfang: | 1394-1403 |
ISSN: |
1526-498X 1526-4998 |
DOI: | 10.1002/ps.4817 |
Zusammenfassung: | <jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND</jats:title><jats:p>20‐Hydroxyecdysone (20E), a crucial insect steroid hormone, can bind to its cognate nuclear receptor composed of ecdysone receptor (EcR) and ultraspiracle (USP) to activate expression of 20E‐response genes, enabling subsequent metamorphosis. In this study, we tried to find out which steroid‐like compounds can block insect metamorphosis effectively and provide useful information for biopesticide study. For this purpose, we screened 126 steroid‐like compounds for possible 20E antagonists using a dual‐luciferase reporter assay with <jats:italic>Drosophila melanogaster</jats:italic> Kc and <jats:italic>Bombyx mori</jats:italic> Bm12 cells.</jats:p></jats:sec><jats:sec><jats:title>RESULTS</jats:title><jats:p>Among 126 steroid‐like compounds, three cucurbitacins (CucB, D and E) were identified as 20E antagonists in both Kc and Bm12 cells. Notably, CucB caused significant molting defects and mortality in both <jats:italic>B. mori</jats:italic> and <jats:italic>D. melanogaster</jats:italic> larvae, and dramatically hindered larval growth of <jats:italic>Helicoverpa armigera</jats:italic> by its anti‐feeding activity.</jats:p></jats:sec><jats:sec><jats:title>CONCLUSION</jats:title><jats:p><jats:italic>In vivo</jats:italic> and <jats:italic>in vitro</jats:italic> experiments demonstrate that CucB acts as a potential insect growth regulator by antagonizing 20E activity and thus blocking molting and metamorphosis induced by 20E signaling. © 2017 Society of Chemical Industry</jats:p></jats:sec> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |