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Th1, Th2, Th17 and Regulatory T Cell Pattern in Psoriatic Patients: Modulation of Cytokines and Gene Targets Induced by Etanercept Treatment and Correlation with Clinical...
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Titel: |
Th1, Th2, Th17 and Regulatory T Cell Pattern in Psoriatic Patients: Modulation of Cytokines and Gene Targets Induced by Etanercept Treatment and Correlation with Clinical Response |
In: | Dermatology, 223, 2011, 1, S. 57-67 |
veröffentlicht: |
S. Karger AG
|
Umfang: | 57-67 |
ISSN: |
1018-8665 1421-9832 |
DOI: | 10.1159/000330330 |
Zusammenfassung: | <jats:p><i>Background:</i> Psoriasis is sustained by pro-inflammatory CD4+ T helper cells mainly belonging to the Th1, Th17 and Th22 lineage. <i>Objective:</i> To identify whether treatment with the anti-tumour-necrosis-factor antagonist etanercept is able to induce significant modulations in transcription factor and cytokine mRNA gene expressions related to the different T cell immune response polarization (Th1, Th2, Th17 and regulatory T cells, T<sub>reg</sub>) and to correlate them with clinical response. <i>Methods:</i> The study population included 19 psoriasis patients treated with etanercept and 19 healthy subjects. Blood samples were collected at baseline and every 4 weeks during treatment. Taqman quantitative real-time polymerase chain reaction was applied to analyse the expression of: Stat-4, T-bet, IL-12p35 and IFN-γ (Th1-related); GATA-3, IL-4 (Th2-related); Stat-3, RORγt, IL-23p19 (Th17-related); Foxp3, IL-2 (T<sub>reg</sub>-related). Flow cytometry was applied to analyse CD4+CD25+<sup>bright</sup>Foxp3+ cells in peripheral blood. <i>Results:</i> Upregulation of Th1 and Th17 and downregulation of T<sub>reg</sub> subsets was found at baseline. The response to etanercept could be associated with a significant reversal of the Th1/Th17 activation, and a concomitant upregulation of Th2 and T<sub>reg</sub> subsets. <i>Conclusion:</i> Our data may contribute to a better understanding of the mechanisms underlying the achievement of clinical response in psoriasis and could be helpful for the identification of early predictive markers of response.</jats:p> |
Format: | E-Article |
Quelle: | S. Karger AG (CrossRef) |
Sprache: | Englisch |