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Cell type-dependent activation of 5-lipoxygenase by arachidonic acid
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Personen und Körperschaften: | , , , , |
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Titel: |
Cell type-dependent activation of 5-lipoxygenase by arachidonic acid |
In: | Journal of Leukocyte Biology, 73, 2003, 1, S. 191-200 |
veröffentlicht: |
Oxford University Press (OUP)
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Umfang: | 191-200 |
ISSN: |
0741-5400 1938-3673 |
DOI: | 10.1189/jlb.0702354 |
Zusammenfassung: | <jats:title>Abstract</jats:title> <jats:p>5-Lipoxygenase (5-LO) is the key enzyme in the biosynthesis of proinflammatory leukotrienes. We show that stimulation of polymorphonuclear leukocytes (PMNL), rat basophilic leukemia (RBL)-1, or transfected HeLa cells with arachidonic acid (AA) caused prominent 5-LO product formation that coincided with the activity of extracellular signal-regulated kinases (ERKs) and p38 mitogen-activated protein kinase. 5-LO product formation in AA-stimulated PMNL and RBL-1 cells was independent of Ca2+. However, in HeLa cells expressing a 5-LO mutant lacking potential 5-LO phosphorylation sites, removal of Ca2+ caused a prominent loss of 5-LO activity. For Mono Mac 6 (MM6) cells, A failed to activate ERKs, and AA-induced 5-LO product formation was only minute. Also, activation of ERKs by phorbol esters did not lead to prominent 5-LO product synthesis. Instead, 5-LO activation in MM6 cells required Ca2+ or alternative signaling pathways induced by hyperosmotic stress. In summary, mechanisms for activation of 5-LO differ considerably between cell types.</jats:p> |
Format: | E-Article |
Quelle: | Oxford University Press (OUP) (CrossRef) |
Sprache: | Englisch |