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Dissecting virulence function from recognition: cell death suppression in Nicotiana benthamiana by XopQ/HopQ1‐family effectors relies on EDS1‐dependent immunity
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Titel: |
Dissecting virulence function from recognition: cell death suppression in Nicotiana benthamiana by XopQ/HopQ1‐family effectors relies on EDS1‐dependent immunity |
In: | The Plant Journal, 91, 2017, 3, S. 430-442 |
veröffentlicht: |
Wiley
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Umfang: | 430-442 |
ISSN: |
0960-7412 1365-313X |
DOI: | 10.1111/tpj.13578 |
Zusammenfassung: | <jats:title>Summary</jats:title><jats:p>Many Gram‐negative plant pathogenic bacteria express effector proteins of the XopQ/HopQ1 family which are translocated into plant cells via the type <jats:styled-content style="fixed-case">III</jats:styled-content> secretion system during infection. In <jats:italic>Nicotiana benthamiana</jats:italic>, recognition of XopQ/HopQ1 proteins induces an effector‐triggered immunity (<jats:styled-content style="fixed-case">ETI</jats:styled-content>) reaction which is not associated with strong cell death but renders plants immune against <jats:italic>Pseudomonas syringae</jats:italic> and <jats:italic>Xanthomonas campestris</jats:italic> pv. <jats:italic>vesicatoria</jats:italic> strains. Additionally, XopQ suppresses cell death in <jats:italic>N</jats:italic>.<jats:italic> benthamiana</jats:italic> when transiently co‐expressed with cell death inducers. Here, we show that representative XopQ/HopQ1 proteins are recognized similarly, likely by a single resistance protein of the <jats:styled-content style="fixed-case">TIR</jats:styled-content>‐<jats:styled-content style="fixed-case">NB</jats:styled-content>‐<jats:styled-content style="fixed-case">LRR</jats:styled-content> class. Extensive analysis of XopQ derivatives indicates the recognition of structural features. We performed <jats:italic>Agrobacterium</jats:italic>‐mediated protein expression experiments in wild‐type and <jats:italic><jats:styled-content style="fixed-case">EDS</jats:styled-content>1</jats:italic>‐deficient (<jats:italic>eds1</jats:italic>) <jats:italic>N</jats:italic>.<jats:italic> benthamiana</jats:italic> leaves, not recognizing XopQ/HopQ1. XopQ recognition limits multiplication of <jats:italic>Agrobacterium</jats:italic> and attenuates levels of transiently expressed proteins. Remarkably, XopQ fails to suppress cell death reactions induced by different effectors in <jats:italic>eds1</jats:italic> plants. We conclude that XopQ‐mediated cell death suppression in <jats:italic>N</jats:italic>.<jats:italic> benthamiana</jats:italic> is due to the attenuation of <jats:italic>Agrobacterium</jats:italic>‐mediated protein expression rather than the cause of the genuine XopQ virulence activity. Thus, our study expands our understanding of XopQ recognition and function, and also challenges the commonly used co‐expression assays for elucidation of <jats:italic>in planta</jats:italic> effector activities, at least under conditions of <jats:styled-content style="fixed-case">ETI</jats:styled-content> induction.</jats:p> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |