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Identification of oral bacteria on titanium implant surfaces by 16S rDNA sequencing
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Titel: |
Identification of oral bacteria on titanium implant surfaces by 16S rDNA sequencing |
In: | Clinical Oral Implants Research, 28, 2017, 6, S. 697-703 |
veröffentlicht: |
Wiley
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Umfang: | 697-703 |
ISSN: |
0905-7161 1600-0501 |
DOI: | 10.1111/clr.12865 |
Zusammenfassung: | <jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>To characterize the profile of microbial communities colonizing titanium implants with different surface treatments after exposure to the oral environment at the genus or higher taxonomic level.</jats:p></jats:sec><jats:sec><jats:title>Material and methods</jats:title><jats:p>Sixteen titanium disks, machined or sandblasted large‐grit and acid‐etched (<jats:styled-content style="fixed-case">SLA</jats:styled-content>), were mounted on removable intraoral splints worn by four patients. After 24 h of intraoral exposure, biofilm samples were collected from disks and supra/subgingival teeth areas. The 16S rDNA genes from each sample were amplified, sequenced with the Miseq Illumina instrument and analyzed.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>A total of 29 genera and seven more inclusive taxa, representing the phyla <jats:italic>Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, Actinobacteria</jats:italic> and candidate division <jats:italic><jats:styled-content style="fixed-case">TM</jats:styled-content>7</jats:italic> were identified in both titanium surfaces and teeth. No differences were found in relation to the operational taxonomic units (<jats:styled-content style="fixed-case">OTU</jats:styled-content>s) and microbial diversity, assessed by Chao 1 and Shannon indices, when comparing <jats:styled-content style="fixed-case">SLA</jats:styled-content> and machined titanium surfaces.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Machined and <jats:styled-content style="fixed-case">SLA</jats:styled-content> surfaces are colonized by similar numbers of prokaryotic OTUs after 24 h of exposure to the oral environment. Higher complexity of the titanium surface topography in the initial phase of biofilm maturation does not seem to significantly influence the colonizing microbiota.</jats:p></jats:sec> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |