Eintrag weiter verarbeiten
Chemical removal of necrotic periodontal ligament on delayed replanted teeth by sodium hypochlorite: morphological analysis and microhardness indentation test of cementum
Gespeichert in:
Personen und Körperschaften: | , , , , |
---|---|
Titel: |
Chemical removal of necrotic periodontal ligament on delayed replanted teeth by sodium hypochlorite: morphological analysis and microhardness indentation test of cementum |
In: | International Endodontic Journal, 49, 2016, 4, S. 393-401 |
veröffentlicht: |
Wiley
|
Umfang: | 393-401 |
ISSN: |
0143-2885 1365-2591 |
DOI: | 10.1111/iej.12467 |
Zusammenfassung: | <jats:title>Abstract</jats:title><jats:sec><jats:title>Aim</jats:title><jats:p>To compare the efficacy of sodium hypochlorite (Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l) used at different concentrations and working times for removing necrotic periodontal ligament (<jats:styled-content style="fixed-case">PDL</jats:styled-content>) from delayed replanted teeth and to observe the effects of Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l on surface structure and microhardness of cementum.</jats:p></jats:sec><jats:sec><jats:title>Methodology</jats:title><jats:p>A total of 88 healthy premolars with a single root extracted for orthodontic purposes were selected and kept dry at room temperature for 1 h. The teeth were divided into 11 groups: group 1 (control): roots were untreated; group 2: necrotic <jats:styled-content style="fixed-case">PDL</jats:styled-content> was removed with gauze; groups 3–11: teeth were immersed in Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l at different concentrations (1, 2.5 and 5.25%) and for different working times (5, 10 and 15 min). The specimens in each group were inspected separately for cementum integrity and the presence of <jats:styled-content style="fixed-case">PDL</jats:styled-content> remnants by histomorphometric analysis, confocal laser scanning microscopy (<jats:styled-content style="fixed-case">CLSM</jats:styled-content>) and scanning electron microscopy (<jats:styled-content style="fixed-case">SEM</jats:styled-content>). Another 14 healthy premolars with roots divided into two pieces were selected for Vickers microhardness indentation tests before and after Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l treatment. The data were analysed statistically using Wilcoxon signed‐rank test of two‐related samples (<jats:italic>P </jats:italic>=<jats:italic> </jats:italic>0.05).</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>In teeth treated with 1% Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l for 15 min or 5.25% Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l for 5 min, the cementum remained morphologically intact without cracks, and <jats:styled-content style="fixed-case">PDL</jats:styled-content> remnants were absent. In the 1% Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l for 15 min group, the microstructure of cementum was arranged more regularly, as observed ×8000 magnification by <jats:styled-content style="fixed-case">SEM</jats:styled-content>. Teeth in each of the other groups displayed cementum damage and/or the presence of <jats:styled-content style="fixed-case">PDL</jats:styled-content> remnants. Microhardness tests revealed that treatment with 1% Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l for 15 min or 5.25% Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l for 5 min significantly decreased microhardness of root cementum (<jats:italic>P </jats:italic><<jats:italic> </jats:italic>0.05).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Use of either 1% Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l for 15 min or 5.25% Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l for 5 min was effective at removing necrotic <jats:styled-content style="fixed-case">PDL</jats:styled-content> from the delayed replanted teeth whilst having a minimal influence on cementum integrity. However, 1% Na<jats:styled-content style="fixed-case">OC</jats:styled-content>l for 15 min was less damaging to cementum.</jats:p></jats:sec> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |