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Raman Studies on Native, Reduced, and Modified Basic Pancreatic Trypsin Inhibitor
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Personen und Körperschaften: | , , |
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Titel: |
Raman Studies on Native, Reduced, and Modified Basic Pancreatic Trypsin Inhibitor |
In: | European Journal of Biochemistry, 50, 1974, 1, S. 129-133 |
veröffentlicht: |
Wiley
|
Umfang: | 129-133 |
ISSN: |
0014-2956 1432-1033 |
DOI: | 10.1111/j.1432-1033.1974.tb03880.x |
Zusammenfassung: | <jats:p>The method of Raman scattering was applied to determine the effect of chemical modification on the conformation of the basic pancreatic trypsin inhibitor in aqueous solution. Cleavage of one of the three disulfide bonds yields no spectral changes except, of course, the manifestation of the reduction, <jats:italic>i.e.</jats:italic> a lower intensity of the disulfide band and the appearance of a line due to the newly formed sulfhydryl groups. S‐alkylation, however, produces several changes in the spectra as compared to the native inhibitor. In the case of the carboxymethyl derivative the amide III band, shifted to lower frequency, is particularly significant. This observation suggests that the inactivity towards trypsin of the reduced carboxymethyl inhibitor is the result of structural changes near the reactive centre of the molecule.</jats:p> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |