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Highly purified CD38+ sub‐populations show no evidence of preferential clonal evolution despite having increased proliferative activity when compared with CD38− sub‐popul...
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Titel: |
Highly purified CD38+ sub‐populations show no evidence of preferential clonal evolution despite having increased proliferative activity when compared with CD38− sub‐populations derived from the same chronic lymphocytic leukaemia patient |
In: | British Journal of Haematology, 142, 2008, 4, S. 595-605 |
veröffentlicht: |
Wiley
|
Umfang: | 595-605 |
ISSN: |
0007-1048 1365-2141 |
DOI: | 10.1111/j.1365-2141.2008.07236.x |
Zusammenfassung: | <jats:title>Summary</jats:title><jats:p>In agreement with a recently published manuscript, this present study demonstrated that CD38<jats:sup>+</jats:sup> sub‐populations had increased proliferative activity as evidenced by higher Ki‐67 expression (<jats:italic>P</jats:italic> < 0·0001). This raised the possibility that the CD38<jats:sup>+</jats:sup> fraction is exposed to an increased risk of clonal evolution. However, serial fluorescence <jats:italic>in situ</jats:italic> hybridisation analysis of highly purified CD38<jats:sup>+</jats:sup> and CD38<jats:sup>−</jats:sup> sub‐populations from individual patients revealed no distinct cytogenetic lesions or evidence of preferential clonal evolution in the CD38<jats:sup>+</jats:sup> fractions when compared with their CD38<jats:sup>−</jats:sup> counter‐parts (<jats:italic>P</jats:italic> = 0·13). Furthermore, telomere length analysis revealed that all of the sub‐populations had similarly short telomeres (<jats:italic>P</jats:italic> = 0·31) and comparably low telomerase (<jats:italic>TERT</jats:italic>) expression (<jats:italic>P</jats:italic> = 0·75) and telomerase activity (<jats:italic>P</jats:italic> = 0·88). Subsequent examination of cell‐sorted CD38<jats:sup>+</jats:sup> and CD38<jats:sup>−</jats:sup> sub‐populations from paired peripheral blood and bone marrow samples taken on the same day showed no significant difference in CD38, Ki‐67, <jats:italic>TERT</jats:italic> expression or telomere lengths, indicating that these chronic lymphocytic leukaemia cells were derived from a single pool trafficking between these two compartments. Taken together, our data show that chronic lymphocytic leukaemia cells derived from bimodal patients all have extensive proliferative histories and have undergone a similar number of cell divisions that is mirrored by the episodic expression of CD38.</jats:p> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |