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Haemorrhage-induced alterations in function and cytokine production of T cells and T cell subpopulations
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Personen und Körperschaften: | , |
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Titel: |
Haemorrhage-induced alterations in function and cytokine production of T cells and T cell subpopulations |
In: | Clinical and Experimental Immunology, 90, 2008, 3, S. 497-502 |
veröffentlicht: |
Oxford University Press (OUP)
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Umfang: | 497-502 |
ISSN: |
0009-9104 1365-2249 |
DOI: | 10.1111/j.1365-2249.1992.tb05873.x |
Zusammenfassung: | <jats:title>SUMMARY</jats:title> <jats:p>Haemorrhage produces alterations in macrophage, T and B cell function. In order to better define the mechanism for the effects of blood loss on immune response, we examined function of and cytokine production by purified T cells, CD4+ and CD8+ subpopulations after blood loss. Whereas T and CD4+ cells from control, unhaemorrhaged animals produced no alteration in proliferation when added to cultures of mitogen-stimulated splenocytes from normal mice, proliferation was decreased when T or CD4+ cells from haemorrhaged mice were included. The addition of CD8+ cells from haemorrhaged animals to mitogen-stimulated cultures reduced proliferation by approximately 50% more than that found when CD8+ cells from control, unhaemorrhaged animals were included. Supernatants of mitogen-stimulated splenocytes from haemorrhaged mice contained significantly less IL-2 and interferon-gamma (IFN-γ) than did those from control, unhaemorrhaged mice. CD4+ populations from haemorrhaged mice produced significantly more IL-10, and significantly less IFN-γ, than did CD4+ cells from control, unhaemorrhaged mice. There were no significant differences in IL-2, IL-4, IL-10 or IFN-γ production by CD8+ cells from haemorrhaged or control mice. The present experiments demonstrate that haemorrhage affects both CD4+ and CD8+ T cell subsets. In particular, haemorrhage appeared to activate CD4+, Th2 cells, with concomitant suppression of the Thl subpopulation. These results provide a mechanism which may contribute to the alterations in cytokine production previously described to occur following blood loss.</jats:p> |
Format: | E-Article |
Quelle: | Oxford University Press (OUP) (CrossRef) |
Sprache: | Englisch |