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The TonB‐dependent ferrichrome receptor FcuA of Yersinia enterocolitica: evidence against a strict co‐evolution of receptor structure and substrate specificity
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Titel: |
The TonB‐dependent ferrichrome receptor FcuA of Yersinia enterocolitica: evidence against a strict co‐evolution of receptor structure and substrate specificity |
In: | Molecular Microbiology, 7, 1993, 3, S. 383-393 |
veröffentlicht: |
Wiley
|
Umfang: | 383-393 |
ISSN: |
1365-2958 0950-382X |
DOI: | 10.1111/j.1365-2958.1993.tb01130.x |
Zusammenfassung: | <jats:title>Summary</jats:title><jats:p>A <jats:italic>Yersinia enterocolitica</jats:italic> receptor mutant was isolated which is impaired in ferrichrome uptake. The receptor‐encoding gene <jats:italic>fcuA</jats:italic> was cloned in <jats:italic>Escherichia coli</jats:italic> K‐12. A <jats:italic>fcuA</jats:italic> mutant of <jats:italic>Y. enterocolitica</jats:italic> could be complemented by the cloned DNA fragment. The FcuA‐encoding region was sequenced and an open reading frame encoding 758 amino acids including a signal sequence of 36 amino acids was found. FcuA shared 34.6% amino acid sequence homology with FatA, the anguibactin receptor of <jats:italic>Vibrio anguillarum</jats:italic>, but only 20.6% homology with FhuA, the ferrichrome receptor of <jats:italic>E. coli</jats:italic> Since the structure of anguibactin differs strongly from that of ferrichrome there seems to be no co‐evolution of receptor structure and substrate specificity.</jats:p><jats:p>The ferrichrome receptors FcuA from <jats:italic>Y. enterocolitica</jats:italic> and FhuA from <jats:italic>E. coli</jats:italic> had slightly different substrate specificities. In contrast to FhuA from <jats:italic>E. coli</jats:italic>, FcuA from <jats:italic>Y. enterocolitica</jats:italic> was more stereoselective and failed to transport enantio ferrichrome. Three additional ferrichrome receptors were cloned from <jats:italic>Pantoea aggiomerans</jats:italic> (formerly <jats:italic>Erwinia herbicola), Salmonella paratyphi</jats:italic> B and <jats:italic>Salmonella typhimurium.</jats:italic> Their substrate specificity was similar but not identical.</jats:p> |
Format: | E-Article |
Quelle: | Wiley (CrossRef) |
Sprache: | Englisch |