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Nucleosome Position-Dependent and -Independent Activation of HIS7 Expression in Saccharomyces cerevisiae by Different Transcri...
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Titel: |
Nucleosome Position-Dependent and -Independent Activation of HIS7 Expression in Saccharomyces cerevisiae by Different Transcriptional Activators |
In: | Eukaryotic Cell, 2, 2003, 5, S. 876-885 |
veröffentlicht: |
American Society for Microbiology
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Umfang: | 876-885 |
ISSN: |
1535-9778 1535-9786 |
DOI: | 10.1128/ec.2.5.876-885.2003 |
Zusammenfassung: | <jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>ARO4</jats:italic> and <jats:italic>HIS7</jats:italic> are two tandemly orientated genes of <jats:italic>Saccharomyces cerevisiae</jats:italic> that are transcribed into the same direction. The <jats:italic>ARO4</jats:italic> terminator and the <jats:italic>HIS7</jats:italic> promoter regions are sensitive to <jats:italic>Micrococcus</jats:italic> nuclease (Mnase) and separated by a positioned nucleosome. The <jats:italic>HIS7</jats:italic> promoter is target for the transcription factors Gcn4p and Bas1p/Bas2p that activate its transcription upon amino acid starvation and purine limitation, respectively. Activation of the <jats:italic>HIS7</jats:italic> gene by Gcn4p overexpression but not by Bas1p/Bas2p releases an ordered nucleosome distribution to yield increased Mnase sensitivity throughout the intergenic region. This remodeling is <jats:italic>SNF2</jats:italic> dependent but mostly <jats:italic>GCN5</jats:italic> independent. Accordingly, <jats:italic>SNF2</jats:italic> is necessary for the Gcn4p-mediated transcriptional activation of the <jats:italic>HIS7</jats:italic> gene. <jats:italic>GCN5</jats:italic> is required for activation upon adenine limitation by Bas1p/Bas2p. Our data suggest that activation of <jats:italic>HIS7</jats:italic> transcription by Gcn4p and Bas1p/Bas2p is supported by a nucleosome position-dependent and -independent mechanism, respectively. Whereas Gcn4p activation causes Swi/Snf-mediated remodeling of the nucleosomal architecture at the <jats:italic>HIS7</jats:italic> promoter, the Bas1p/Bas2p complex presumably activates in combination with Gcn5p-dependent histone acetylation. </jats:p> |
Format: | E-Article |
Quelle: | American Society for Microbiology (CrossRef) |
Sprache: | Englisch |