ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice

Bibliographic Details
Authors and Corporations: Yan, Song
Title: ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice
In: Plant Molecular Biology, 68, 2008, 3, p. 239-250
published:
Springer Netherlands
Physical Description:239-250
ISSN/ISBN: 0167-4412
1573-5028
EISSN:1573-5028
Summary:Leaves, the collective organ produced by the shoot apical meristem (SAM), are polarized along their adaxial–abaxial axis. In this study, we characterized two rice (<Emphasis Type="Italic">Oryza sativa</Emphasis>) allelic rolled-leaf mutants, <Emphasis Type="Italic">rolled leaf 9-1 </Emphasis>(<Emphasis Type="Italic">rl9-1</Emphasis>) and <Emphasis Type="Italic">rl9-2</Emphasis>, which display very similar phenotypes with completely adaxialized leaves and malformed spikelets. We cloned the <Emphasis Type="Italic">RL9</Emphasis> gene by way of a map-based cloning strategy. Molecular studies have revealed that <Emphasis Type="Italic">RL9</Emphasis> encodes a GARP protein, an orthologue of <Emphasis Type="Italic">Arabidopsis</Emphasis><Emphasis Type="Italic">KANADIs</Emphasis>. <Emphasis Type="Italic">RL9</Emphasis> is mainly expressed in roots, leaves, and flowers. The transient expression of a <Emphasis Type="Italic">RL9</Emphasis>–GFP (green fluorescent protein) fusion protein has indicated that <Emphasis Type="Italic">RL9</Emphasis> protein is localized in the nucleus, suggesting that <Emphasis Type="Italic">RL9</Emphasis> acts as a putative transcription factor.
Type of Resource:E-Article
Source:Springer Journals
sid-105-col-springerjournals
Language: English